Shellab BACTROX User Manual download pdf (Page 19)

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Bactrox Operations Manual 19

18. BACTROX OPERATION TIPS

Gas Source:

Sheldon Manufacturing, Inc. recommends an two separate tanks of 100% CO, ,

and 100% Nitrogen for Hypoxic applications.

These mixtures are available marked “NON-FLAMMABLE”.

Two national sources of gas are AIRGAS, PRAXAIR, and Air Liquid.

How much gas will a

Bactrox chamber use?

Gas usage is dependent upon three things; the number of times a user

enters their chamber daily, operating technique, and the number of PASS

BOX purge cycles.

Our product literature states the PASS BOX uses approximately 45

seconds of gas to purge the oxygen to appropriate hypoxic levels.

Methods to Conserve Gas:

Users can utilize the PASS BOX as a holding station. As samples are

received they can be stored in a hypoxic environment by placing them in

the PASS BOX and activating either the automatic or manual PASS BOX

cycle.

1. Users should evaluate and try to limit the number of times the PASS

BOX is cycled. For example, placing samples in the PASS BOX

once in the morning and once in the afternoon as opposed to four

times a day is desirable.

2. Gas is also conserved by bringing samples in through the sleeve

system. Easy samples to transport through the sleeves are transport

tubes as well as plates that are sealed.

3. Good sleeve system technique.

When a user is in the “Working Area” they should be aware of the

effects their movements have on gas consumption.

Gas consumption can be reduced by balancing arm movements.

For example, when extending the right arm into the chamber, bring

the left arm close to the front of the chamber, thus balancing the

pressure and reducing gas displaced by area.

Aborting PASS BOX PURGE

CYCLE:

The automatic PASS BOX can be aborted by pressing and holding the

“START PASS BOX PURGE” button for five (5) seconds. If a user forgets

to place a sample in the PASS BOX the cycle can be aborted in order to

eliminate the unnecessary use of gas.

Chamber Checklist:

It is recommended that customers review a checklist before they enter the

Bactrox Chamber. The checklist serves as a reminder of supplies and

samples they need to bring into the unit.

Common items listed on a checklist are: re-activated anatox, samples and

loops. The checklist can be placed on the front Plexiglas panel.

Size of Sleeve Cuffs:

It is critical that the sleeve cuffs seal around the user’s arm during chamber

operation to ensure the desired hypoxic environment.

The location that the cuffs seal on the user’s arm is determined by

individual preference.

The size of the cuffs that come standard with the Bactrox Chamber is

medium, size 8 glove. Other sizes are available; large size 9, and small

size 6 ½.

Replacement of Cuffs:

When and how often the cuffs on the sleeve need to be replaced depends

on customer care and technique. Typically the cuffs are replaced about

every three months.

Customers should watch for small holes that can appear where the cuffs

are placed on the “ring cuff”. If holes are present the cuffs should be

replaced to avoid gas leak. Watches and jewelry can damage the cuffs

and are not recommended to be worn during chamber use.

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Shellab BACTROX User Manual Page 19

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