Shellab BACTROX User Manual Page 10

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Bactrox Operations Manual 10
point temperature. Allow the incubator at least 24 hours to stabilize. Then re-calibrate the digital
display to your reference thermometer, follow the calibration instructions. This incubator was
calibrated at the factory at 37
0
C.
7.2 Setting Incubator Safety: First, set the control to the desired incubator temperature and then the
INCUBATOR SAFETY to its maximum position. Allow 24 hours for stabilization before
proceeding.
7.3 If, after 24 hours the temperature is not at desired level, adjust the SET TEMPERATURE up or
down using the arrows until the precise desired temperature is achieved.
7.4 When stabilization at the desired temperature has been achieved, turn the SET OVER
TERMPERATURE control knob counter-clockwise until the OVER TEMP ACTIVATED light
comes on and audible alarm is activated. Next, carefully turn the SET OVER TERMPERATURE
knob clockwise until the light is just off. The safety is now set; the OVER TEMP ACTIVATED
light should remain OFF during normal operation.
7.5 NOTE: An accurate thermometer should be used inside the incubator as a reference when
setting and calibrating the incubator temperature.
7.6 It is a good idea to mark the SET OVER TERMPERATURE label with the desired setting or
position as a backup in case the knob is moved accidentally. If the OVER TEMP ACTIVATED
light is on and audio alarm is activated at any time, check the SET TEMPERATURE controller
setting to be sure that it is not set above the SET OVER TERMPERATURE setting.
7.7 Alarm Controls and Display: The temperature control, O
2
, and the CO
2
control are both
equipped with visual and audible alarms.
• Temperature Alarms: The temperature control has alarm indicators for high and low conditions
that are activated whenever the actual temperature is 1°C above or below the set point.
O
2
Alarms: The O
2
controller has alarm indicators for high and low alarm conditions that are
activated whenever the actual O
2
% is 1% above or below the set point.
• CO
2
Alarms: The CO
2
controller has alarm indicators for high and low alarm conditions that are
activated whenever the actual CO
2
% is 1% above or below the set point.
• Audible Alarms: Both controls have audible alarms that are activated when either of the HIGH
or LOW indicators is activated.
7.8 Muting Audible Alarms: The audible alarms can be muted for a single alarm occurrence by
pressing and holding down EITHER the UP or DOWN button for several seconds until the alarm
mutes. There is a built in delay of approximately 15 minutes on the occurrence of a LOW alarm.
This time delay prevents the audible alarm from activating every time the door is opened and the
temperature and CO
2
drops.
8. CO
2
CONTROL CALIBRATION
8.1 Manufacturer’s Recommendation for Monitoring CO
2
Levels: CO
2
sensors are factory calibrated
and, under normal circumstances, need no calibration. It is recommended that the accuracy of
your CO
2
control system be monitored by measuring the actual CO
2
concentration on a weekly
basis with a Fyrite or other measuring device. This should be done when the chamber has not
been opened for an extended period of time, i.e. after the weekend, and should only take 1-2
hours.
8.2 Setting the CO
2
Control: Attach the supply hose from the CO
2
tank to the Bactrox CO
2
inlet fitting
and turn on the CO
2
supply. Set the CO
2
control to the desired set point using the up and down
arrows. The CO
2
level comes from the factory preset to OFF.
8.3 Adjusting CO
2
Display: After the Bactrox has had several hours to stabilize at the desired CO
2
set point and measure the actual CO
2
% with a Fyrite gas analyzer. If there is any difference
between the Fyrite gas analyzer and the display, use the procedure described in section 7 page
14 for calibrating CO
2
. See Section 9 Page 19 (next page) for Fyrite use and instruction.
NOTE: When using the Fyrite gas analyzer, insure that gas is not being injected while the reading is
being taken. Always change the CO
2
set point to 0.0 prior to taking the sample and change the set point
back to the desired value after the use of the Fyrite gas analyzer is finished.
8.4 Using a FYRITE to Measure CO
2
Levels
A Bacharach FYRITE CO
2
Gas Analyzer is recommended to measure CO
2
concentrations in the Bactrox chamber. This test instrument is not supplied with the
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